qPCR

Description of qPCR

Development of an easy sample preparation procedure to extract DNA sample compatible with qPCR from the typology of raw specimen available in the project and provided to ST by other partners.

Optimization of qPCR reaction mixtures on the platform starting from applications’ targets (including primers and probes) suggested by participating partners and using commercially available product. Fine-tuning of qPCR thermal protocol and optical parameters and validation: optimization tests will be performed analyzing samples processed with the developed sample preparation procedure and using the defined qPCR reaction mixtures.

The objective will be to determine the best reaction parameters, in terms of maximum sensitivity and specificity, in detecting the EWA-BELT target applications with the BIO2BIT® platform. After the optimization phase, performances of the test will be validated against a gold-standard qPCR instrument.